Scientist I - Epigenetic Editing Technologies

OPPORTUNITY Mammoth is hiring a Scientist I to contribute to R&D projects within the Editing Technologies team. The Editing Technologies team develops next-generation CRISPR tools for therapeutic genome editing applications, with scope spanning from technological ideation to translational proof-of-concept. The successful candidate will work as an individual contributor in designing novel epigenetic editing tools and evaluating their performance in mammalian cells.  Strength in wet lab execution and experimental design along with deep scientific understanding of epigenetics and epigenetic editing will be critical to the role. The candidate will work within a core team of epigenetic tool developers and collaborate cross-functionally with a diverse technical staff with expertise in disease biology, bioinformatics, protein engineering, high-throughput screening/automation, reagent production, and in vivo evaluation. This is a fantastic opportunity to impact human health by making CRISPR tools that cure genetic disease.

KEY RESPONSIBILITIES

• Contribute creatively to the design of new epigenetic editing tools through ideation and literature review
• Contribute to the development of scalable assays for measuring gene expression, epigenetic state, and/or cellular phenotype.
• Plan, design and execute experiments for CRISPR tool evaluation
• Document experiments in electronic laboratory notebooks and present results at internal meetings
• Disclose inventions to the IP team for patenting
• Other duties as assigned

REQUIRED QUALIFICATIONS

• PhD in Molecular Biology, Biochemistry, Biomolecular Engineering or a related discipline. For non-PhD candidates with a Bachelor's degree, a minimum of 5 years of professional experience is required
• 2+ years of hands-on experience designing and refining fusion proteins for CRISPR or other DNA binding proteins (ZNF, TALE)
• 2+ years of hands-on experience designing, executing, and analyzing CRISPRa/i or epigenetic editing experiments in mammalian cells
• 1+ years hands-on experience in at least two of the following areas:
• Epigenetic characterization assays including bisulfite sequencing (targeted or whole genome), EM-seq, ChIP-qPCR, and/or ChIP-seq
• Flow cytometry and/or fluorescence-activated cell sorting (FACS)
• Transcript/protein quantification assays including RT-qPCR; d/ddPCR, RNA-seq, ELISA, and/or MSD
• Automated methods for high-throughput molecular/cell biology workflows

PREFERRED QUALIFICATIONS

• 1+ years experience with mammalian cell line engineering
• 1+ years experience with developing pooled screening approaches for high-throughput tool evaluation
• 1+ years experience working with disease-specific cell models and optimizing nucleic acid delivery via lipofection, nucleofection, and/or viral transduction
• Working proficiency with common bioinformatics tools (Benchling, JMP, SQL, R, Python)

BENEFITS

• Company-paid health/vision/dental benefits
• Unlimited vacation and generous sick time
• Company-sponsored meals and snacks
• Wellness, caregiver and ergonomics benefits
• 401(k) with company matching