Arvind Phansatkar has a background in microbiology, holding an M.Sc. from Mumbai University, which was completed in 2000. Arvind served as a Research Fellow at Lokmanya Tilak Municipal Medical College from April 1996 to December 2000, focusing on rapid diagnosis of tuberculosis using PCR for a thesis. In February 2011, Arvind briefly worked at GlaxoSmithKline as a Microbiologist and Manufacturing Team member.
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